Subproject 15

Ecotoxicological analysis in surface water

Subproject 15 foresees the carrying out of ecotoxicological tests to verify the acute and chronic toxicity of surface water samples collected along the Paraopeba River Basin. In addition, this subproject aims to evaluate the route of exposure to contaminants and the potability of surface water collected upstream and downstream from the collapse of Dam I of Mina do Córrego Feijão in relation to ecotoxicological tests.

This objective is divided into:

  • Perform acute toxicity tests in relation to the bioluminescence emitted by Allivibrio fischeri and Zebrafish (Danio rerio) for samples collected upstream and downstream from the breaking point of Dam I of the “Córrego Feijão” mine;
  • Perform chronic toxicity tests with Ceriodaphnia spp. and Raphidocelis subcapitata (formerly Pseudokirchneriella subcapitata) for samples collected upstream and downstream from the breakpoint of Dam I of the “Córrego Feijão” mine;
  • Perform acute and chronic toxicity tests on newly hatched Zebrafish larvae (Danio rerio) for samples collected upstream and downstream from the breakpoint of Dam I of the “Córrego Feijão” mine;
  • Carry out mutagenicity and genotoxicity tests for samples collected upstream and downstream from the breaking point of Dam I of the “Córrego Feijão” mine;
  • Prepare technical reports (partial and final) containing the results obtained in the proposed ecotoxicological tests and relate them in an integrated manner with the results obtained for the physicochemical characterization.

Methodological steps

Step 1: Formalization of the contract, purchases and mobilization of the team working on the project

Step 2: Training of fellows to carry out ecotoxicological tests and instrument calibration

Step 3: Carrying out ecotoxicological tests with samples collected in the River Basin

          3.1. Selection of Sample Points

          3.2. Required sample volume

          3.3 Carrying out ecotoxicological tests

          3.4 Acute toxicity analysis with Allivibrio fischeri

          3.5 Chronic toxicity analysis with Ceriodaphinia spp

          3.6 Analysis of chronic toxicity with Raphidocelis subcapitata

          3.7 Acute toxicity tests with adult Danio rerio (Zebrafish)

          3.8 Acute and chronic toxicity in Zebrafish larvae (Danio rerio)

          3.9 Genotoxicity and mutagenicity analyzes

Step 4: Integrated analysis of results and reporting

 

Meet our team!

Subproject Timeline 23

Month 1 (11/2020)
Month 2
Month 3
Month 4
Month 5
Month 6
Month 7
Month 8
Month 9
Month 10
Month 11
Month 12 (11/2021)

• Formalization of the contract, hiring of scholarship holders and purchases;
• Scholarship training

• Scholarship training ;
• Carrying out ecotoxicological tests;

• Testing ecotoxicological;

• Testing ecotoxicological;

• Testing ecotoxicological;

• Testing ecotoxicological;

• Testing ecotoxicological;
• Preparation of partial report;

• Testing ecotoxicological;

• Testing ecotoxicological;

• Testing ecotoxicological;
• Preparation of final report;

• Testing ecotoxicological;
• Preparation of final report;

• Testing ecotoxicological;
• Presentation and preparation of final report;

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